Pricing Overview
We have experience with a wide range of techniques and the ability to accommodate a variety of projects. As a guide, we have listed pricing below for some of the more common types of sequencing runs. To obtain a quote or discuss a project type that is not listed here, please contact us.
If you would prefer to prepare your own libraries, we also offer a variety of sequencing options. User-prepared libraries must be run separately in their own lane or flowcell.
| NovaSeq X Plus Run Type | Flowcell | Expected Reads/Run | Cost/Run |
|---|---|---|---|
| 100 cycles (100SR, PE50) | 1.5B | 1.6 billion reads | $2,800 |
| 100 cycles (100SR, PE50) | 5B | 5 billion reads | $6,100 |
| 100 cycles (100SR, PE50) | 10B | 10 billion reads | $6,800 |
| 100 cycles (100SR, PE50) | 25B | 26 billion reads | $14,900 |
| 200 cycles (PE100, PE75) | 1.5B | 1.6 billion reads | $3,700 |
| 200 cycles (PE100, PE75) | 5B | 5 billion reads | $7,800 |
| 200 cycles (PE100, PE75) | 10B | 10 billion reads | $8,200 |
| 200 cycles (PE100, PE75) | 10B (single lane) | 1.25 billion reads | $1,100 |
| 200 cycles (PE100, PE75) | 25B | 26 billion reads | $19,600 |
| 300 cycles (PE150) | 1.5B | 1.6 billion reads | $4,200 |
| 300 cycles (PE150) | 5B | 5 billion reads | $8,300 |
| 300 cycles (PE150) | 10B | 10 billion reads | $9,900 |
| 300 cycles (PE150) | 25B | 26 billion reads | $23,500 |
| 600 cycles (PE300) | 1.5B | 1.6 billion reads | $6,500 |
| MiSeq Run Type | Expected Reads/Run | Cost/Run |
|---|---|---|
| MiSeq v3, 600 cycle kit | Up to 25 million reads | $2,500 |
| MiSeq v3, 150 cycle kit | Up to 25 million reads | $1,400 |
| MiSeq v2, 500 cycle kit | Up to 15 million reads | $1,900 |
| MiSeq v2, 300 cycle kit | Up to 15 million reads | $1,600 |
| MiSeq v2, 50 cycle kit | Up to 15 million reads | $1,200 |
| MiSeq v2 Micro, 300 cycle kit | Up to 4 million reads | $850 |
| MiSeq v2 Nano, 500 cycle kit | Up to 1 million reads | $800 |
| MiSeq v2 Nano, 300 cycle kit | Up to 1 million reads | $600 |
Our bulk RNAseq and miRNAseq pricing includes library prep and sequencing as described. Our DNA library prep pricing only includes library prep, as the required sequencing depth will be project specific. The figures shown in the table below are the cost per sample, where there are two tiers depending on the number of samples submitted.
| Bulk Sequencing Service | Sequencing Configuration | <32 Samples | 32+ Samples |
|---|---|---|---|
| mRNAseq (polyA selection) | 18-20 M reads/sample, 100PE | $320 | $280 |
| Total RNAseq | 25-30 M reads/sample, 100PE | $330 | $290 |
| Paxgene RNAseq (+globin depletion) | 25-30 M reads/sample, 100PE | $450 | $400 |
| miRNAseq | 10-15 M reads/sample, 100PE | $380 | $340 |
| DNA library prep | Library prep only | $100 | $90 |
The ENBRC Genomics Core will offer the following services on the PacBio Vega:
- Premade SMRTbell library sequencing
- Whole genome sequencing (Mammalian)
- Amplicon sequencing (coming soon!)
- Kinnex bulk full-length RNA sequencing (coming soon!)
- Kinnex single-cell full-length RNA sequencing (10x Genomics cDNA) (coming soon!)
Custom services under development:
- Rhesus macaque immunoglobulin H chain sequencing – near full-length Adaptive Immune Receptor Repertoire sequencing (FLAIRR-seq)
- SIV Single Molecule Real Time Capture (SIV SMRTcap)
Please contact us if you are interested in any applications of PacBio long-read sequencing.
Our single cell library prep and sequencing services are priced separately as the number of cells captured will affect the required amount of sequencing. Our standard gene expression assay is run using the 5' 10X Genomics method, and the VDJ and Feature Barcode library preps are available as add-on services to a gene expression capture.
| Nuclei Isolation | Cost/Sample |
|---|---|
| Nuclei Isolation for ATAC only assays | $170 |
| Nuclei Isolation for GEX only or Multiome assays | $240 |
| Single Cell/Nucleus Capture, Library Prep & Sequencing | Pricing Unit | Unit Cost |
|---|---|---|
| Universal Gene Expression Assays | ||
| Gene Expression Capture & Library Prep | Price/capture (up to 20,000 cells) | $1,650 |
| GEM-X Single Cell 5’ Chip Kit v3/3’ Chip Kit v4 | Price/chip (up to 8 captures per chip) | $297 |
| Gene Expression Capture & Library Prep 4-plex (OCM) | Price/capture (up to 20,000 cells) | $1,790 |
| GEM-X OCM 5’ Chip Kit v3 4-plex/3’ Chip Kit v4 4-plex | Price/chip (up to two 4-plex captures per chip) | $285 |
| Sequencing of Gene Expression Libraries – 50,000 reads/cell | Price/1,000 cells | $140 |
| Sequencing of Gene Expression Libraries – 30,000 reads/cell | Price/1,000 cells | $84 |
| VDJ Library Prep (add-on) | Price/capture | $250 |
| Feature Barcode Library Prep (add-on) | Price/capture | $250 |
| Sequencing of VDJ or Feature Barcode Libraries | Price/1,000 cells | $24 |
| Epi Chromatin Assays | ||
| ATAC Capture & Library Prep | Price/capture (up to 10,000 nuclei) | $1,850 |
| Next GEM Chip H Single Cell Kit | Price/chip (up to 8 captures per chip) | $295 |
| Multiome (ATAC + Gene Expression) Capture & Library Prep | Price/capture (up to 20,000 nuclei) | $2,700 |
| GEM-X Epi Multiome Chip Kit | Price/chip (up to 8 captures per chip) | $297 |
| Sequencing of ATAC Libraries – 25,000 reads/cell | Depends on project | |
| Flex Gene Expression Assays | ||
| Flex Single-plex Capture & Library Prep | Price/capture (up to 20,000 cells) | $980 |
| Flex Multi-plex | Please contact us for a quote | |
| GEM-X Flex Gene Expression Chip Kit | Price/chip (up to 8 captures per chip) | $297 |
| Sequencing of Flex Gene Expression Libraries – 20,000 reads/cell | Price/1,000 cells | $35 |
Our spatial transcriptomics library prep and sequencing services are priced separately as the % of the capture area covered by tissue will affect the required amount of sequencing. Charges for the histology component are not shown, these will be invoiced directly from the histology/imaging core you choose to work with.
| Spatial Transcriptomics | Pricing Unit | Unit Cost |
|---|---|---|
| RNA extraction and DV200/RIN determination | Price/Sample from tissue block | $33 |
| Visium HD CytAssist Library Prep (WT Panel/3’ Assay 6.5 x 6.5 mm) | Price/Visium HD slide (with two 6.5 x 6.5 mm areas) | $6,800 |
| Visium HD CytAssist Library Prep (WT Panel 11 x 11 mm) | Price/Visium HD slide (with two 11 x 11 mm areas) | $9,300 |
| Sequencing of FFPE Visium HD WT Assay Libraries (6.5 x 6.5 mm) | Price/10% of capture area (275 M reads for 100% covered) | $77 |
| Sequencing of FF Visium HD WT Assay Libraries (6.5 x 6.5 mm) | Price/10% of capture area (700 M reads for 100% covered) | $196 |
| Sequencing of FxF Visium HD WT Assay Libraries (6.5 x 6.5 mm) | Price/10% of capture area (500 M reads for 100% covered) | $140 |
| Sequencing of FF Visium HD 3’ Assay Libraries (6.5 x 6.5 mm) | Price/10% of capture area (550 M reads for 100% covered) | $154 |
| Sequencing of FFPE Visium HD WT Assay Libraries (11 x 11 mm) | Price/10% of capture area (825 M reads for 100% covered) | $168 |
| Sequencing of FF Visium HD WT Assay Libraries (11 x 11 mm) | Price/10% of capture area (2100 M reads for 100% covered) | $588 |
| Sequencing of FxF Visium HD WT Assay Libraries (11 x 11 mm) | Price/10% of capture area (1500 M reads for 100% covered) | $420 |
These prices include QC via Qubit and TapeStation or Fragment Analyzer. *Please note that extraction projects have a six sample minimum.
| RNA Extraction Services | Starting Sample Type | Cost/Sample |
|---|---|---|
| Qiagen RNeasy Mini* | Cells/tissue snap frozen or in RLT | $24 |
| Qiagen RNeasy Micro* | Cells/tissue snap frozen or in RLT | $29 |
| Qiagen miRNeasy Mini/Micro* | Cells/tissue snap frozen or in QIAzol | $26 |
| MagMAX PAXgene Blood RNA* | Blood in PAXgene RNA tubes | $27 |
We are happy to provide quality control services for various samples that you have prepared yourself. **Please note that Bioanalyzer projects have a six sample minimum.
| QC Service Type | Cost/Sample or Library |
|---|---|
| Bioanalyzer (High Sensitivity DNA)** | $110 (cost per chip) |
| TapeStation (RNA, RNA-HS, D5000-HS, D1000-HS, Genomic DNA) | $8 |
| Fragment Analyzer (RNA, RNA-HS, DNA-HS NGS Fragment (1-6000bp)) | $6 |
| Qubit (DNA or RNA) | $3 |
| Library QC, pooling and normalization | $14 |
| Library Pool QC (KAPA Library Quantification Kit - ROX Low) | $40 |
We offer a variety of bioinformatics services to support data generated in our genomics core. This can include a basic alignment and annotation of your sequencing data to produce gene count tables and bam files, or further statistical analyses to identify differentially expressed genes and activated pathways. As the scope of each project can vary widely, we encourage you to contact us for a quote.
Last Updated: 2026-08-03