Next Generation Sequencing


We offer next generation sequencing services on our Illumina NovaSeq X Plus, Illumina NovaSeq 6000 and Illumina MiSeq. The combination of these systems provides us with a wide degree of flexibility in terms of read length and depth, allowing us to quickly sequence small amplicon-based sequencing projects as well as larger whole-genome scale projects.

Overview of Illumina Sequencing by Synthesis Workflow | XLEAP SBS chemistry

NovaSeq X Plus

The NovaSeq X series has many improvements on its predecessor, the NovaSeq 6000, including exceptional speed (higher throughput ultrahigh-density flow cells and reduced run times), data quality and accuracy (advanced chemistry and ultrahigh-resolution optics) and a significant reduction in sequencing reagent costs.

The NovaSeq X Plus system is powered by Illumina XLEAP-SBS chemistry – a faster, higher fidelity, and more robust advancement to proven Illumina sequencing by synthesis (SBS) chemistry. The onboard DRAGEN platform speeds up computing and facilitates faster data transfers.

Four flow cell types are currently available with the X series,1.5B, 5B, 10B, and 25B, which provides the broadest output range. The NovaSeq X Plus System generates from 165 Gb/1.6 B reads to 8 Tb/26 B reads of data in single flow cell mode. In dual flow cell mode, output can be up to 16 Tb and 52 B reads.

Flow Cell Type1.5B5B10B25B
Lanes per Flow Cell2888
Clusters PF1.6 billion5 billion10 billion26 billion
Available Run Configurations
PE50, SR100 (100 cycle)YesYesYesYes
PE100 (200 cycle)YesYesYesYes
PE150 (300 cycle)YesYesYesYes
PE300 (600 cycle)YesNoNoNo

MiSeq

The MiSeq is a smaller unit used primarily for small whole-genome sequencing, targeted gene (amplicon) sequencing, immune repertoire sequencing, and 16s metagenomics sequencing. We also offer a variety of flow cell types for the MiSeq to better serve a range of sequencing depths and project sizes.

MiSeq flow cell specifications and run configurations
Flow Cell Typev2 Nanov2 Microv2v3
Clusters PFUp to 1 millionUp to 4 millionUp to 15 millionUp to 25 million
Available Run Configurations
PE75, SR150 (150 cycle)NoNoNoYes
PE150 (300 cycle)YesYesYesNo
PE250 (500 cycle)YesNoYesNo
PE300 (600 cycle)NoNoNoYes